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Acrylamide-ES ELISA Test Kit with 0.03ppb High Sensitivity for 1.5 Hours Detection Time and 96T Specification

Name: Acrylamide-ES ELISA Test Kit with 0.03ppb High Sensitivity for 1.5 Hours Detection Time and 96T Specification
Brand: Maoming Chenhui Biotechnology Co., Ltd.
SKU: CH520363
Price: 48.00 USD
Availability: InStock

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Acrylamide-ES ELISA Test Kit with 0.03ppb High Sensitivity for 1.5 Hours Detection Time and 96T Specification

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Détails sur le produit:

Lieu d'origine:	Chine
Nom de marque:	Chenhui
Numéro de modèle:	CH520363

Conditions de paiement et expédition:

Quantité de commande min:	5 trousses
Prix:	negotiable
Détails d'emballage:	Couleur Emballage
Délai de livraison:	5-8 jours ouvrables
Conditions de paiement:	T/T
Capacité d'approvisionnement:	1000 trousses

Contact

Acrylamide-ES ELISA Test Kit with 0.03ppb High Sensitivity for 1.5 Hours Detection Time and 96T Specification

description de

Rapide:	10-30min	Récupération élevée:	80 à 105%
Haute sensibilité:	00,03 ppm	Temps de détection:	1,5 heures
Température de stockage:	2-8°C	Spécification:	96t

Acrylamide-ES ELISA Test Kit Used For Detecting Instant Coffee, Fried Foods, And Baked Foods

Acrylamide-ES ELISA Test Kit (CH520363)

Intended Use: For the detection of Acrylamide in ground coffee and fried food using the Acrylamide-ES ELISA Kit.

Range of Detection: This method detects 15 ppb to 1,200 ppb in matrix.

Materials Required (Not Provided)

Pipette(s) capable of delivering 2 uL-20 uL, 20 uL-200 uL and 100 uL-1,000 uL
Vortex mixer
Analytical balance
50 mL conical tubes
Deionized or distilled water (boiling)
Overhead mixer/rotator
Fume hood
Ammonium hydroxide (ACS Grade 28-30%)
Centrifuge capable of 4,000 rpm
Biotage ISOLUTE® SLE+ columns, 15 mL (Biotage PN 820-0290-D)
Biotage ISOLUTE® column adapter (Biotage PN 120-1102)
10 mL syringe
20 mL glass vials with Teflon-lined caps
Ethylene glycol (99% purity)
Ethyl acetate (HPLC grade)
Tetrahydrofuran (99% purity)
Nitrogen
Heating block or water bath
Glass graduated cylinder
Glass rod
Ring stand and clamps
Acrylamide-ES ELISA Kit

Notes and Precautions

4.1 Pipettes should be calibrated prior to performing the extraction and/or dilutions.

4.2 Pipetting volumes less than 10 uL can lead to error due to the small volume being dispensed.

4.3 This procedure is for use with ground coffee only. Samples of coffee beans must be ground before extraction to produce accurate results.

4.4 Prepare the elution solution by mixing ethyl acetate and tetrahydrofuran at a 1:1 ratio.

Example: Add 100 mL of ethyl acetate and 100 mL of tetrahydrofuran to a glass container and mix thoroughly.

4.5 Ammonium hydroxide, ethylene glycol, ethyl acetate, tetrahydrofuran and boiling water must be handled with care. Wear protective clothing (gloves, glasses, etc.). Avoid contact with skin and mucous membranes. If contact occurs, wash with copious amounts of water and seek medical attention.

4.6 Using the glass rod, gently press down on the top frit of the SLE+ columns to ensure the frit is fully adhering to the column bed. Proper contact is necessary to maintain uniform flow through the column bed.

4.7 When extracting decaffeinated ground coffee only use 2 mL of ammonium hydroxide (see Step 5.4).

Procedure

5.1 Weigh 1 g of ground coffee sample into a 50 mL conical tube.

5.2 Add 20 mL of boiling water (distilled or deionized) to the sample. Cap the tube tightly.

5.3 Place the sample on an overhead mixer and rotate at 50% speed-setting for 30 minutes.

5.4 Place the sample in a ventilated hood and add 2.5 mL of ammonium hydroxide to the sample (note: only use 2 mL for decaffeinated coffee).

5.5 Place the sample on the overhead mixer and rotate at 50% speed-setting for 30 minutes.

5.6 Centrifuge the sample for 5 minutes at 4,000 rpm.

5.7 Add 1.5 mL of the supernatant to the SLE+ column. Use the column adapter and 10 mL syringe to apply gentle, positive pressure until all of the supernatant passes onto the column bed. DO NOT FORCE THE SUPERNATANT THROUGH THE COLUMN BED—the sample must remain on the column. If the sample flows through the column, the column must be discarded and a new column should be used with a fresh aliquot of supernatant.

Produit Tag:

Personne à contacter: Tina

Téléphone: +8615818799493

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